Yesterday I received another INADEQUATE spectrum, this time of sucrose. The S/N is still high enough to make my filter unnecessary. If I play with the contour plot all the noise disappears while the 12 carbon atoms and their 10 bonds remain. Only a spurious peak remains at the coordinates 103.7;-22.9. I have not received the 1-D external projection, so I created it artificially. The spectral width is the same in both dimension (instead of being doubled for the DQF axis). The consequence is that two cross-peaks fall just on the boundary and are partially folded. This is the spectrum:
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I have applied the filter with the same parameters used for the cholesterol (C-C coupling; linewidths in the two dimensions) while the threshold corresponds to the above plot. The result is perfect. All the cross-peaks are resolved and they are all present. Nothing else survives.
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This time all the peaks are regular anti-phase doublets. The Js are generally larger than in cholesterol.
Click on the thumbnails to see the full-size pictures. There is an expansion to help counting the correct number of cross-peaks.
Do you want to send another spectrum? I can clean it for free. Remember to enclose the 13-C of the same sample.
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